Phospho ampk antibody cst

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August undefined, 2024

WebAll lanes : Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) at 1/500 dilution Lane 1 : HUVEC cell extract untreated Lane 2 : HUVEC cell extract treated with UV Predicted band size: 64 kDa. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) WebThe Phospho-AMPKalpha (Thr172) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to PRKAA1, and PRKAA2. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: Western Blot.

(2532) AMPKα Antibody - Cell Signaling Technology - CiteAb

WebFeb 26, 2024 · home > human > AMPK antibody This is a Validated Antibody Database (VAD) review about human AMPK, based on 7 published articles ( read how Labome selects the articles ), using AMPK antibody in all methods. It is aimed to help Labome visitors find the most suited AMPK antibody. WebOct 19, 2024 · Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine. graham swafford attorney jasper tennessee

Anti-phospho-AMPK α (Thr172) Antibody 07-681

WebImmunogen. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human AMPKα. Antibodies are … Web使用 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb（上）或 AMPKα Antibody #2532（下），对未经处理或经寡霉素处理 (0.5 µM) 的 C2C12 细胞提取物进行蛋白质印迹分析。使用 Phospho-AMPKα (T172) (40H9) Rabbit mAb，对对照（左）或经苯乙双胍处理（右）的石蜡包埋的 NCI-H228 细胞沉淀物进行免疫组织化学分析。使用 Phospho-AMPKα (Thr172) … WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse … china industrial hot air dryer

Ming-Mu-Di-Huang-Pill Activates SQSTM1 via AMPK-Mediated ... - Hindawi

Phospho-AMPK-alpha (Thr172) Polyclonal Antibody - Thermo …

WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 950 publications. Validated for WB, IP, IHC. Highly specific and rigorously validated in-house, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit Monoclonal Antibody (CST #4376) is ready to ship. WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). graham sutherland winston churchillWebJul 1, 2024 · Adenosine 5′-monophosphate (AMP)–activated protein kinase (AMPK), a critical cellular energy sensor that increases life span in multiple species [reviewed in ()], is one of the factors required for the enhanced longevity caused by inhibition of mitochondrial respiration in C. elegans (5–7).AMPK is activated by an elevated AMP/adenosine 5′ … grahams vintage port 2007

"WebPhospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen … " - Phospho ampk antibody cst

Phospho ampk antibody cst

Anti-phospho-AMPK α (Thr172) Antibody 07-681 - EMD Millipore

WebThis antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: … WebSep 4, 2014 · Western blot analysis using a phospho-specific antibody revealed a significant increase in phospho-Thr184-AMPK levels in head tissue lysates from ElavGS>UAS-AMPK flies upon RU486 ... Primary antibody, rabbit anti-DILP2 (a generous gift from Dr. Seung Kim), or anti-GFP (Cell Signaling Technology; D5.1, XP) was added 1:250 and incubated …

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WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. WebPhospho-AMPK-alpha (Thr172) Antibody (620-120) in WB. Western Blot: The whole cell lysate derived from EGF stimulated A431 immunoblotted by Rabbit anti AMPK alpha 1 …

WebAMPK is a heterotrimer consisting of a catalytic α-subunit and two regulatory subunits, β and γ. Each subunit exists as multiple isoforms (α 1, α 2, β 1, β 2, γ 1, γ 2, and γ 3 ), giving 12 different possible combinations of holoenzyme with different tissue distribution and subcellular localization ( 2 , 3 ). WebPhospho-AMPKα (Thr172) (40H9) Rabbit mAb detects endogenous AMPKα1 only when phosphorylated at threonine 183 and endogenous …

WebAMPK signaling pathway; 浏览更多通路>> ... Phospho-CDK9 (Thr186) Antibody detects endogenous levels of CDK9 only when phosphorylated at Thr186. RRID: AB_2844504 引用格式: Affinity Biosciences Cat# AF4440, RRID:AB_2844504. 偶联: Unconjugated. WebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material.

WebWestern blot analysis of Phospho-IRE1 alpha (Ser724) in HeLa cells. Samples were incubated in Phospho-IRE1 alpha (Ser724) polyclonal antibody (Product # PA1-16927) using a dilution of 2.0 µg/mL followed by an anti-rabbit HRP secondary antibody. Treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR.

WebThis antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. The AMPKalpha Antibody was generated using … china industrial magnetron equipment factoryWebAMP-Activated Protein Kinases / analysis AMP-Activated Protein Kinases / metabolism* Amino Acid Sequence Animals Caco-2 Cells Cells, Cultured Hepatocytes / metabolism* Humans Male Mass Spectrometry Membrane Proteins / analysis Membrane Proteins / metabolism* Mice Mice, Inbred C57BL Mitochondrial Proteins / analysis grahamsville weather mapWebDescription: The AMPK and ACC Antibody Sampler Kit provides an economical means to investigate energy homeostasis and fatty acid synthesis within the cell. The kit includes … grahamsville reformed church grahamsville nyWebPolyclonal Antibody for studying AMPK-alpha1 (Ser496) phosphate. Cited in 30 publications. Validated for Western Blotting. Highly specific and … graham swannell actorWebetary methodology from Cell Signaling Technology for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid graham swamp trail festWebAMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) are meta- bolic kinases that co-ordinate nutrient supply with cell growth. AMPK negatively regulates mTORC1, and mTORC1 reciprocally phosphorylates S345/7 in both AMPK a-isoforms. graham swannell playwrightWebAMP-activated protein kinase (AMPK) is a key cellular energy sensor and regulator of metabolic homeostasis. Although it is best known for its effects on carbohydrate and lipid … graham swamp palm coast fl