WebNov 15, 2011 · Blots were incubated with a rabbit polyclonal anti-CYP2A5 antibody (1:2000 dilution; sourced from Dr. Juvonen, University of Kuopio, Finland) for 2 h. Membranes were then incubated for 1 h with horseradish peroxidase-conjugated goat anti-rabbit IgG (1:3000 dilution). After further washing with phosphate-buffered saline, blots were incubated in ... WebAnti-CYP2A5 Antibody. Mouse liver CYP2A5 protein was purified by octylsepharose and hydroxyapatite chromatography as previ-ouslydescribed.27 …
Heme and Heme Biosynthesis Intermediates Induce Heme
WebAntibodies and biochemical assay kits. Anti-CYP2A5 antibody was a gift from Dr. Risto Juvonen (Department of Pharmacology and Toxicology, University of Kuopio, Kuopio, Finland) and anti-CYP2E1 was a gift from Dr. Jerome Lasker (Hackensack Biomedical Research Institute, Hackensack, NJ). The other antibodies and assay kits are WebSep 1, 2000 · Expression of the CYP2A13 protein was confirmed by immunoblot analysis with an antibody to mouse CYP2A5 . As shown in the inset of Fig. 2, a single band of the expected size was detected in the microsomal fraction of CYP2A13-expressing Sf9 cells, but not in control cells. The recombinant CYP2A13 migrated to the same position as did a … cu chulainn persona 5 strikers
Recombinant Anti-CYP2C19 antibody [EPR6576] …
WebDec 8, 2024 · Cyp2a5 represents the first example of a P450 gene regulated by oxidative stress through nuclear factor(erythroic-derived 2)-like 2 wild-type mice. induction of … WebImmunopurified proteins, pulled down with anti-CYP2A5 from 500 g of lung microsomes or 20 to ϳ 25 fmol of recombinant CYP2A6 or CYP2A13 (both in insect Sf9-cell microsomes), were analyzed on ... WebMar 15, 2024 · Cyp2a5 knockout (Cyp2a5 −/−) mice develop more severe alcoholic fatty liver than Cyp2a5 +/+ mice. Fibroblast growth factor 21 (FGF21), a PPARα-regulated … cuchulainn teamwear