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Ai9 tdtomato reporter mice

WebJan 5, 2024 · The Ai9 reporter mice harbors a lox-STOP-lox (LSL)-tdTomato reporter knockin at the Rosa26 locus 11 (Figure 1). Using two sgRNAs flanking LSL, Cas9 will delete the STOP cassette by NHEJ, resulting in tdTomato expression. 12 This system allows direct detection of cells edited by CRISPR in vivo. WebApr 13, 2024 · Here, we develop one tamoxifen inducible RBPMS-tdTomato reporter mouse line, which allowed the precise identification and tracking of the RBPMS expressing cells during embryogenesis and adulthood. With the help of this mouse line, it is achievable to location the RGCs during development, simulate RGC related degenerative disease, …

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WebThe Jackson Laboratory tdtomato cre reporter ai9 mouse line Tdtomato Cre Reporter Ai9 Mouse Line, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebApr 11, 2024 · We therefore examined sections from brain and spinal cord of NPFF Cre mice crossed with the Ai9 reporter line, in which Cre-dependent excision of a STOP … roman defensive fortification https://surfcarry.com

007905 - B6;129S6-Gt(ROSA)26Sor /J

WebOct 27, 2024 · Expression of the Cre-recombinase activity was evaluated using the reporter mouse strain Ai9 at pre-hearing, hearing onset, and post-hearing stages. Accordingly, heterozygous Nefl CreER/ + ;iDTR +/- mice were treated with tamoxifen on postnatal days 1-5 (P1-5), followed by diphtheria toxin (DT) or vehicle injection on P7, P14, and P21 to ... WebApr 6, 2024 · Methods UCP1-Cre mice crossed to Ai14-tdTomato and Ai9-tdTomato reporter mice were used to explore the tissue distribution of Cre recombinase and Ucp1 mRNA in various tissues. Web007909 B6.Cg- Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG … roman deadlift with kettlebell

007905 - Ai9 or Ai9(RCL-tdT) Strain Details - The …

Category:Conditional gene targeting using UCP1-Cre mice directly …

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Ai9 tdtomato reporter mice

007909 - Ai9 or Ai9(RCL-tdT) Strain Details

WebThe Jackson Laboratory ai9 r26r tdtomato cre reporter mice. Ai9 R26r Tdtomato Cre Reporter Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz … WebPlasmid pDEST-Cas9-tdTomato from Dr. Hideyuki Okano's lab contains the insert Cas9 and is published in PLoS One. 2024 Aug 27;14(8):e0221164. doi: …

Ai9 tdtomato reporter mice

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WebStudies in our own laboratory using the Ai9 TdTomato reporter line have shown Dmp1-Cre expression in some osteoblasts, skeletal muscle, a subset of cells in the marrow, stomach, small intestine, skin, brain, eye and an even more restricted subset of cells in the lung and kidney (see Fig 2). WebDec 20, 2009 · Strong tdTomato native fluorescence in Ai9 and Ai14 allowed direct visualization of fine structural details, such as dendritic spines and thin axons, of diverse …

WebBars = 200 μm. d Lung and kidney showed a low level of TdTomato reporter expression in 10-kb Dmp1-Cre(+) mice compared to Cre(−) littermates. The expression was seen in only a small subset of ... WebAi9 Cre Reporter Mouse Strain and Study Plans for AAV Local and Systemic Delivery (A) Ai9 Cre reporter mouse strain with tdTomato expression induced by Cre recombinase …

WebJan 1, 2024 · Here, we examined the distribution of Cre and UCP1 throughout the body in UCP1-Cre reporter mice. Methods UCP1-Cre mice crossed to Ai14-tdTomato and Ai9-tdTomato reporter mice were used to explore the tissue distribution of Cre recombinase and Ucp1 mRNA in various tissues. Web目的构建稳定表达的tdTomato转基因小鼠并建系,观察其细胞及组织的荧光表达水平以及其干细胞共培养后的荧光表达水平。方法使用Gateway clone技术和DNA显微注射法构建含有tdTomato表达载体的受精卵,移植至假孕母鼠体内,待其自然生产。使用双向鉴定法鉴定并挑取饲养阳性小鼠并建系。

Web口腔医学小站 口腔文献阅读与资源分享. 2024年4月3日 发表者 kimi1006 暂无评论. Programmable protein delivery with a bacterial contractile injection system Nature

WebNov 8, 2024 · We used immunofluorescence for PV on tissue from mice expressing tdTomato in PV-INs to address the specificity and efficacy of the PV-IRES-Cre mouse line in the mouse PER. ... 743 tdTomato-expressing neurons, and 719 double labeled neurons. Similar to our results with the Ai9 reporter line, the specificity was very high (96.85 ± 1%, … roman defeatWebJun 22, 2024 · All mice carry the Ai9 TdTomato reporter. Inset shows a higher power view of TdTomato positive areas in brain. Bars = 200 μm. d Lung and kidney showed a low level of TdTomato reporter expression in 10-kb Dmp1-Cre (+) mice compared to Cre (−) littermates. The expression was seen in only a small subset of cells within the tissue. roman deck mount faucethttp://www.dentalearner.com/page/2 roman delay wipesroman demeter crosswordWebDownload scientific diagram Development of 5-HT Cre*Ai9 tdTomato reporter mice designed to express tdTomato fluorescent protein only in 5-HT neuron. roman defensive battlesWebDec 13, 2024 · Ai9 Cre Reporter Mouse Strain and Study Plans for AAV Local and Systemic Delivery (A) Ai9 Cre reporter mouse strain with tdTomato expression induced by Cre recombinase expressed by a viral vector, which excises the stop cassette upstream of the tdTomato expression cassette. roman deity marsWebAi9 mouse (BCM) Official Name: B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J: Species: Mouse: Type: Animal: Description: Ai9 mouse has a loxP-flanked STOP … roman delight eastpoint mall