WebJan 5, 2024 · The Ai9 reporter mice harbors a lox-STOP-lox (LSL)-tdTomato reporter knockin at the Rosa26 locus 11 (Figure 1). Using two sgRNAs flanking LSL, Cas9 will delete the STOP cassette by NHEJ, resulting in tdTomato expression. 12 This system allows direct detection of cells edited by CRISPR in vivo. WebApr 13, 2024 · Here, we develop one tamoxifen inducible RBPMS-tdTomato reporter mouse line, which allowed the precise identification and tracking of the RBPMS expressing cells during embryogenesis and adulthood. With the help of this mouse line, it is achievable to location the RGCs during development, simulate RGC related degenerative disease, …
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WebThe Jackson Laboratory tdtomato cre reporter ai9 mouse line Tdtomato Cre Reporter Ai9 Mouse Line, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebApr 11, 2024 · We therefore examined sections from brain and spinal cord of NPFF Cre mice crossed with the Ai9 reporter line, in which Cre-dependent excision of a STOP … roman defensive fortification
007905 - B6;129S6-Gt(ROSA)26Sor /J
WebOct 27, 2024 · Expression of the Cre-recombinase activity was evaluated using the reporter mouse strain Ai9 at pre-hearing, hearing onset, and post-hearing stages. Accordingly, heterozygous Nefl CreER/ + ;iDTR +/- mice were treated with tamoxifen on postnatal days 1-5 (P1-5), followed by diphtheria toxin (DT) or vehicle injection on P7, P14, and P21 to ... WebApr 6, 2024 · Methods UCP1-Cre mice crossed to Ai14-tdTomato and Ai9-tdTomato reporter mice were used to explore the tissue distribution of Cre recombinase and Ucp1 mRNA in various tissues. Web007909 B6.Cg- Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG … roman deadlift with kettlebell